Cowhage, cowitch, Bengal bean, buffalo bean, kaw ai, kratzbohnen, mucuna, nescafé, sea beans, true sea-bean, aatmaguptaa, konchh, poonaikkaali.
Common names in Spanish:
Pica-pica, chiporro, guisante negro, chhican, ojo de venado (Quattrocchi, 2012; Rehm, 1994).
Where is it found?
This annual climbing shrub is native to Asia and grows in India, Pakistan, Bangladesh, among many other countries. It is now cultivated in Australia (Khare, 2016).
Parts of the plant used:
Principally the seed, along with the root, leaves, and pods.
How is it used?
The seed and seed pods are taken internally to treat various ailments (Quattrocchi, 2012, Ghani, 2003).
What is it used for?
Velvet bean is a tropical legume that is traditionally used in Asia for controlling blood pressure (Chaudhary et al.,2015). In ancient Indian medicine, the plant has been used for a plethora of diseases and ailments, including the following: It has been used for the management of male infertility, nervous disorders, and as an aphrodisiac. The seeds have medicinal properties and have been used in the major traditional medical systems of India (Ayurveda, Siddha, and Unani-Tibb) for many centuries. The seeds are rich in the compound known as L-DOPA, which is found principally in the seeds, but also in the pods, leaves, and roots. The plant’s leaf extracts and seeds show promise for the treatment of Parkinson’s disease, male infertility, and nervous disorders, mainly due to their antioxidant (free radical scavenging) effects. Parts of the plant may also serve as aphrodisiacs. (Khare, 2016, 2007; Lampariello et al., 2012).
The pods have anthelminthic action (to eliminate intestinal worms). A tea made from the root is sweetened with honey is used to treat cholera. A leaf paste is applied topically for skin ulcers (Ghani, 2003).
The seeds can lower blood glucose levels, the hairs on the pods are taken as a syrup to kill intestinal worms, the crushed seeds combined with molasses are ingested to kill gastrointestinal parasites, a decoction of the plant is drunk to treat fever and dysentery, the roasted seeds are eaten as an aphrodisiac, and a seed decoction is taken as a tea to regularize menstrual cycles. The root and seeds have a tonic action. The roots have a stimulant, purgative, diuretic, and astringent action. A root decoction is drunk to relieve kidney ailments. The root, tied to the arm of a male partner, is said to prolong sexual intercourse (Quattrocchi, 2012; Khare, 2007).
Studies by Herrera-Chalé et al., (2016 a, b) suggested that the Velvet bean’s bioactive peptides can lower cholesterol levels and also have a blood-thinning (anticoagulant) effect. Additionally, the plant’s antioxidant bioactive compounds may also lower blood pressure. For these reason, the authors suggested that the plant might have a beneficial use as a nutraceutical supplement.
A study evaluated the effects on laboratory animals (rabbits) of an herbal combination that included Velvet bean (Mucuna pruriens). The results of the study showed that there was no mortality or any evidence of systemic toxicity, following a 2-month administration of the herbal combination (Ahmed et al., 2015).
A study employing Velvet bean standardized seed extracts concluded that the seed is a potential ACE (angiotensin-converting enzyme) inhibitor that needs to be further studied as a potentially effective option for lowering blood pressure (Chaudhary et al., 2015).
Kumar et al., (2016) studied the antiproliferative actions of an isoquinoline alkaloid isolated from the seeds of the velvet bean in hepatic carcinoma cells. The authors concluded that some of the bioactive ingredients (alkaloids) in Velvet bean were active against liver cancer cells and could be useful for a future treatment of liver cancer.
Safety / Precautions
- Avoid use during pregnancy (Gardner and McGuffin, 2013).
- The safety for use during lactation has not been established.
- Patients taking antidiabetic medications should consult with a healthcare provider before taking preparations containing velvet bean.
- The seed pods are covered with hairs that can cause itching and severe irritation to the skin (dermatitis) (Quattrocchi, 2012).
Before you decide to take any medicinal herb or herbal supplement, be sure to consult with a health care professional first. Avoid self-medication and self-diagnosis: Always be on the safe side!
Ahmed S, Khan RA, Feroz Z. Assessment of sub-chronic, hematological and histopathological toxicities of a herbal combination. Pak J Pharm Sci. 2015; 28(6):2153-60.
Chaudhary SK, De A, Bhadra S, Mukherjee PK. Angiotensin-converting enzyme (ACE) inhibitory potential of standardized Mucuna pruriens seed extract.
Pharm Biol. 2015;53(11):1614-20. doi: 10.3109/13880209.2014.996820.
Gardner Z, McGuffin M (Editors). Botanical Safety Handbook 2nd ed.
Boca Raton, FL; CRC Press; 2013; pp. 584-586.
Ghani A. Medicinal Plants of Bangladesh; Chemical Constituents and Uses 2nd ed.
Dhaka, Bangladesh: Asiatic Society of Bangladesh; 2003; pp. 308-309.
a Herrera Chalé F, Ruiz Ruiz JC, Betancur Ancona D, Acevedo Fernández JJ, Segura Campos MR. The hypolipidemic effect and antithrombotic activity of Mucuna pruriens protein hydrolysates. Food Funct. 2016; 7(1):434-44. doi: 10.1039/c5fo01012h.
b Herrera-Chalé F, Ruiz-Ruiz JC, Betancur-Ancona D, Segura-Campos MR. Potential Therapeutic Applications of Mucuna pruriens Peptide Fractions Purified by High-Performance Liquid Chromatography as Angiotensin-Converting Enzyme Inhibitors, Antioxidants, Antithrombotic and Hypocholesterolemic Agents. J Med Food. 2016 ;19(2):187-95. doi: 10.1089/jmf.2015.0098.
Khare C P. Ayurvedic Pharmacopeial Plant Drugs.
Boca Raton, FL: CRC Press; 2016; pp. 373-374.
Khare C P. Indian Medicinal Plants: An Illustrated Dictionary.
New Delhi, India: Springer-Verlag; 2007; p. 424-425.
Kumar P, Rawat A, Keshari AK, Singh AK, Maity S, De A, Samanta A, Saha S. Antiproliferative effect of isolated isoquinoline alkaloid from Mucuna pruriens seeds in hepatic carcinoma cells. Nat Prod Res. 2016; 30(4):460-3. doi: 10.1080/14786419.2015.1020489.
Lampariello LR, Cortelazzo A, Guerranti R, Sticozzi C, Valacchi G.
The Magic Velvet Bean of Mucuna pruriens. J Tradit Complement Med. 2012; 2(4):331-9.
Quattrocchi, U. World Dictionary of Medicinal and Poisonous Plants, Vol 4.
Boca Raton, FL: CRC Press; 2012; pp. 208-209.
Rehm S. Multilingual Dictionary of Agronomic Plants.
Norwich, MA: Kluwer Academic Publishers; 1994; pp. 124-125.
Velvet seed Cowhage, cowitch, Bengal bean, buffalo bean, kaw ai, kratzbohnen, mucuna, nescafé, sea beans, true sea-bean, aatmaguptaa, konchh, poonaikkaali. Common names in Spanish:
The Magic Velvet Bean of Mucuna pruriens
Lucia Raffaella Lampariello
1 Department of Chemistry, University of Siena, Italy.
2 Department of Internal Medicine, Endocrine-Metabolic Sciences and Biochemistry, University of Siena, Italy.
2 Department of Internal Medicine, Endocrine-Metabolic Sciences and Biochemistry, University of Siena, Italy.
3 Department of Life Science and Biotechnologies, University of Ferrara, Ferrara, Italy
3 Department of Life Science and Biotechnologies, University of Ferrara, Ferrara, Italy
4 Department of Food and Nutrition, Kyung Hee University, Seoul, South Korea
Mucuna pruriens (Fabaceae) is an established herbal drug used for the management of male infertility, nervous disorders, and also as an aphrodisiac. It has been shown that its seeds are potentially of substantial medicinal importance. The ancient Indian medical system, Ayurveda, traditionally used M. pruriens, even to treat such things as Parkinson’s disease. M. pruriens has been shown to have anti-parkinson and neuroprotective effects, which may be related to its anti-oxidant activity. In addition, anti-oxidant activity of M. pruriens has been also demonstrated in vitro by its ability to scavenge DPPH radicals and reactive oxygen species. In this review the medicinal properties of M. pruriens are summarized, taking in consideration the studies that have used the seeds extracts and the leaves extracts.
The genus Mucuna, belonging to the Fabaceae family, sub family Papilionaceae, includes approximately 150 species of annual and perennial legumes. Among the various under-utilized wild legumes, the velvet bean Mucuna pruriens is widespread in tropical and sub-tropical regions of the world. It is considered a viable source of dietary proteins (Janardhanan et al., 2003; Pugalenthi et al., 2005) due to its high protein concentration (23–35%) in addition its digestibility, which is comparable to that of other pulses such as soybean, rice bean, and lima bean (Gurumoorthi et al., 2003). It is therefore regarded a good source of food.
The dozen or so cultivated Mucuna spp. found in the tropics probably result from fragmentation deriving from the Asian cultigen, and there are numerous crosses and hybrids (Bailey and Bailey, 1976). The main differences among cultivated species are in the characteristics of the pubescence on the pod, the seed color, and the number of days to harvest of the pod. “Cowitch” and “cowhage” are the common English names of Mucuna types with abundant, long stinging hairs on the pod. Human contact results in an intensely itchy dermatitis, caused by mucunain (Infante et al., 1990). The nonstinging types, known as “velvet bean” have appressed, silky hairs.
The plant M. pruriens, widely known as “velvet bean,” is a vigorous annual climbing legume originally from southern China and eastern India, where it was at one time widely cultivated as a green vegetable crop (Duke, 1981). It is one of the most popular green crops currently known in the tropics; velvet beans have great potential as both food and feed as suggested by experiences worldwide. The velvet bean has been traditionally used as a food source by certain ethnic groups in a number of countries. It is cultivated in Asia, America, Africa, and the Pacific Islands, where its pods are used as a vegetable for human consumption, and its young leaves are used as animal fodder.
The plant has long, slender branches; alternate, lanceolate leaves; and white flowers with a bluish-purple, butterfly-shaped corolla. The pods or legumes are hairy, thick, and leathery; averaging 4 inches long; are shaped like violin sound holes; and contain four to six seeds. They are of a rich dark brown color, and thickly covered with stiff hairs. In India, the mature seeds of Mucuna bean are traditionally consumed by a South Indian hill tribe, the Kanikkars, after repeated boiling to remove anti-nutritional factors. Most Mucuna spp. exhibit reasonable tolerance to a number of abiotic stresses, including drought, low soil fertility, and high soil acidity, although they are sensitive to frost and grow poorly in cold, wet soils (Duke, 1981). The genus thrives best under warm, moist conditions, below 1500 m above sea level, and in areas with plentiful rainfall. Like most legumes, the velvet bean has the potential to fix atmospheric nitrogen via a symbiotic relationship with soil microorganisms.
Mucuna spp. have been reported to contain the toxic compounds L-dopa and hallucinogenic tryptamines, and anti-nutritional factors such as phenols and tannins (Awang et al., 1997). Due to the high concentrations of L-dopa (4–7%), velvet bean is a commercial source of this substance, used in the treatment of Parkinson’s disease. The toxicity of unprocessed velvet bean may explain why the plant exhibits low susceptibility to insect pests (Duke, 1981). Velvet bean is well known for its nematicidic effects; it also reportedly possesses notable allelopathic activity, which may function to suppress competing plants (Gliessman et al., 1981).
Despite its toxic properties, various species of Mucuna are grown as a minor food crop. Raw velvet bean seeds contain approximately 27% protein and are rich in minerals (Duke, 1981). During the 18 th and 19 th centuries, Mucuna was grown widely as a green vegetable in the foothills and lower hills of the eastern Himalayas and in Mauritius. Both the green pods and the mature beans were boiled and eaten. In Guatemala and Mexico, M. pruriens has for at least several decades been roasted and ground to make a coffee substitute; the seeds are widely known in the region as “Nescafé,” in recognition of this use.
Mucuna pruriens as a traditional medicine
M. pruriens is a popular Indian medicinal plant, which has long been used in traditional Ayurvedic Indian medicine, for diseases including parkinsonism (Sathiyanarayanan et al., 2007). This plant is widely used in Ayurveda, which is an ancient traditional medical science that has been practiced in India since the Vedic times (1500–1000 BC). M. pruriens is reported to contain L-dopa as one of its constituents (Chaudhri, 1996). The beans have also been employed as a powerful aphrodisiac in Ayurveda (Amin, 1996) and have been used to treat nervous disorders and arthritis (Jeyaweera, 1981). The bean, if applied as a paste on scorpion stings, is thought to absorb the poison (Jeyaweera, 1981).
The non-protein amino acid-derived L-dopa (3,4-dihydroxy phenylalanine) found in this under-utilized legume seed resists attack from insects, and thus controls biological infestation during storage. According to D’Mello (1995), all anti-nutritional compounds confer insect and disease resistance to plants. Further, L-dopa has been extracted from the seeds to provide commercial drugs for the treatment of Parkinson’s disease. L-Dopa is a potent neurotransmitter precursor that is believed, in part, to be responsible for the toxicity of the Mucuna seeds (Lorenzetti et al., 1998). Anti-epileptic and anti-neoplastic activity of methanol extract of M. pruriens has been reported (Gupta et al., 1997). A methanol extract of MP seeds has demonstrated significant in vitro anti-oxidant activity, and there are also indications that methanol extracts of M. pruriens may be a potential source of natural anti-oxidants and anti-microbial agents (Rajeshwar et al., 2005).
All parts of M. pruriens possess valuable medicinal properties and it has been investigated in various contexts, including for its anti-diabetic, aphrodisiac, anti-neoplastic, anti-epileptic, and anti-microbial activities (Sathiyanarayanan et al., 2007). Its anti-venom activities have been investigated by Guerranti et al. (2002) and its anti-helminthic activity has been demonstrated by Jalalpure (2007). M. pruriens has also been shown to be neuroprotective (Misra and Wagner, 2007), and has demonstrated analgesic and anti-inflammatory activity (Hishika et al., 1981).
Functional components of Mucuna pruriens
In addition to the low levels of sulfur-containing amino acids in M. pruriens seeds, the presence of anti-physiological and toxic factors may contribute to a decrease in their overall nutritional quality. These factors include polyphenols, trypsin inhibitors, phytate, cyanogenic glycosides, oligosaccharides, saponins, lectins, and alkaloids. Polyphenols (or tannins) are able to bind to proteins, thus lowering their digestibility. Phenolic compounds inhibit the activity of digestive as well as hydrolytic enzymes such as amylase, trypsin, chymotrypsin, and lipase. Recently, phenolics have been suggested to exhibit health related functional properties such as anti-carcinogenic, anti-viral, anti-microbial, anti-inflammatory, hypotensive, and anti-oxidant activities.
Trypsin inhibitors belong to the group of proteinase inhibitors that include polypeptides or proteins that inhibit trypsin activity. Tannins exhibit weak interactions with trypsin, and thus also inhibit trypsin activity. Phytic acid [myoinositol-1,2,3,4,5,6-hexa(dihydrogen phosphate)] is a major component of all plant seeds, which can reduce the bioavailability of certain minerals such as zinc, calcium, magnesium, iron, and phosphorus, as well as trace minerals, via the formation of insoluble complexes at intestinal pH. Phytate-protein complexes may also result in the reduced solubility of proteins, which can affect the functional properties of proteins.
Cyanogenic glycosides are plant toxins that upon hydrolysis, liberate hydrogen cyanide. The toxic effects of the free cyanide are well documented and affect a wide spectrum of organisms since their mode of action is inhibition of the cytochromes of the electron transport system (Laurena et al., 1994). Hydrogen cyanide (HCN) is known to cause both acute and chronic toxicity, but the HCN content of M. pruriens seeds is far below the lethal level. Janardhan et al. (2003) have investigated the concentration of oligosaccharides in M. pruriens seeds, and verbascose is reportedly the principal oligosaccharide therein (Siddhuraju et al., 2000). Fatty acid profiles reveal that lipids are a good source of the nutritionally essential linoleic and oleic acids. Linoleic acid is evidently the predominant fatty acid, followed by palmitic, oleic, and linolenic acids (Mohan and Janardhanan, 1995; Siddhuraju et al., 1996). The nutritional value of linoleic acid is due to its metabolism at tissue levels that produce the hormone-like prostaglandins. The activity of these prostaglandins includes lowering of blood pressure and constriction of smooth muscle. Phytohemagglutinins (lectins) are substances possessing the ability to agglutinate human erythrocytes.
The major phenolic constituent of M. pruriens beans was found to be L-dopa (5%), along with minor amounts of methylated and non-methylated tetrahydroisoquinolines (0.25%) (Sidhuraju et al., 2001; Misra and Wagner, 2004). However, in addition to L-dopa, 5-indole compounds, two of which were identified as tryptamine and 5-hydroxytryptamine, were also reported in M. pruriens seed extracts (Tripathi and Updhyay, 2001). Mucunine, mucunadine, prurienine, and prurieninine are four alkaloids that have been isolated from such extracts (Mehta and Majumdar, 1994). The chemical structures of some of these compounds are shown in Figure 1 .
Chemical structures of some proteic and non-proteic compounds contained in Mucuna pruriens
a) N-terminal amino acid sequences of proteins at positions (A) 1, 2, and 4 after gel separation, which are identical, and (B) at position 3, which is identical to those in A with regard to the first 10 aa, and (C) positions 5, 6 and 7, which differ from A in only in 3 aa.
b) The reaction representing the two steps involved in the formation of dopamine from l-tyrosine and the non-protein amino acid L-dopa (the main phenolic compound contained in MP)
c) Chemical structures of d-chiro-inositol and its two galacto-derivatives, O-α-d-galactopyranosil-(1→2)-d-chiro-inositol (FP1) and O-α-dgalactopyranosil-(1→6)-O-α-d-galactopyranosil-(1→2)-d-chiro-inositol (FP2), present in MP seeds.
Pharmacological effects of Mucuna pruriens extracts
All parts of the Mucuna plant possess medicinal properties (Sathiyanarayanan and Arulmozhi, 2007). In vitro and in vivo studies on M. pruriens extracts have revealed the presence of substances that exhibit a wide variety of pharmacological effects, including anti-diabetic, anti-inflammatory, neuroprotective and anti-oxidant properties, probably due to the presence of L-dopa, a precursor of the neurotransmitter dopamine (Misra and Wagner, 2007). It is known that the main phenolic compound of Mucuna seeds is L-dopa (approximately 5%) (Vadivel and Pugalenthi, 2008). Nowadays, Mucuna is widely studied because L-dopa is a substance used as a first-line treatment for Parkinson’s disease. Some studies indicate that L-dopa derived from M. pruriens has many advantages over synthetic L-dopa when administered to Parkinson’s patients, as synthetic L-dopa can have several side effects when used for many years.
In small amounts (approximately 0.25%) L-dopa corresponds to methylated and non-methylated tetrahydroisoquinoline (Siddhuraju and Becker, 2001; Misra and Wagner, 2004). These substances are present in the Mucuna roots, stems, leaves, and seeds. Other substances are present in different parts of the plant, among which are N,N-dimethyl tryptamine and some indole compounds (Tripathi and Updhyay, 2001). Alcoholic extracts of the seeds were shown to have potential anti-oxidant activity in in vivo models of lipid peroxidation induced by stress (Tripathi and Updhyay, 2001). On the other hand, Spencer et al. (1996) have reported that the pro-oxidant and anti-oxidant actions of L-dopa and its metabolites promote oxidative DNA damage and could also be harmful to tissues damaged by neurodegenerative diseases, namely parkinsonism. Moreover, a study using in vitro models revealed that L-dopa significantly increases the levels of oxidized glutathione in rat brain striatal synaptosomes (Spina et al., 1988). The observed depletion of reduced glutathione (GSH) could be due to the generation of reactive semiquinones from L-dopa (Spencer et al, 1995).
Protective effect of Mucuna pruriens seeds against snake venom poisoning
M. pruriens is one of the plants that have been shown to be active against snake venom and, indeed, its seeds are used in traditional medicine to prevent the toxic effects of snake bites, which are mainly triggered by potent toxins such as neurotoxins, cardiotoxins, cytotoxins, phospholipase A2 (PLA2), and proteases (Guerranti et al., 2002). In Plateau State, Nigeria, the seed is prescribed as a prophylactic oral anti-snakebite remedy by traditional practitioners, and it is claimed that when the seeds are swallowed intact, the individual is protected for one full year against the effects of any snake bite (Guerranti et al., 2001). The mechanisms of the protective effects exerted by M. pruriens seed aqueous extract (MPE), were investigated in detail, in a study involving the effects of Echis carinatus venom (EV) (Guerranti et al., 2002). In vivo experiments on mice showed that protection against the poison is evident at 24 hours (short-term), and 1 month (long term) after injection of MPE (Guerranti et al., 2008). MPE protects mice against the toxic effects of EV via an immune mechanism (Guerranti et al., 2002). MPE contains an immunogenic component, a multiform glycoprotein, which stimulates the production of antibodies that cross-react with (bind to) certain venom proteins (Guerranti et al., 2004). This glycoprotein, called gpMuc (see Table 1 ), is composed of seven different isoforms with molecular weights between 20.3 and 28.7 kDa, and pI between 4.8 and 6.5 (Di Patrizi et al., 2006).
Pharmacological activity of Mucuna pruriens and its compounds
It is likely that one or more gpMuc isoform is analogous in primary structure to venom PLA2. The presence of at least one shared epitope has been demonstrated with regard to MP seeds and snake venom. These cross-reactivity data explain the mechanism of the long-term protection conferred by MP, and confirm that certain plant species contain PLA2-like proteins, which are beneficial for plant growth, and are involved in important processes (Lee et al., 2005). In addition, MP seeds contain protein and non-protein components that are able to directly inhibit the activity of proteases and PLA2, and are responsible for short-term protection. In fact, MPE contains protease inhibitors that are active against snake venom, in particular a gpMuc isoform sequence also found in a “Kunitz type” trypsin inhibitor contained in soy. Two-dimensional gel electrophoresis has been used to separate the seven gpMuc isoforms, in order to perform N-terminal analysis of each individual isoform. The sequences obtained are shown in Figure 1 . According to their sequences, we can group the isoforms at positions 1, 2, and 4 on the gel, which are identical in 12/12 aa. The isoform at position 3 is identical to those aforementioned, with regard to the first 10 aa, and those at positions 5, 6, and 7 differ from those at positions 1,2 and 4 by just 3 aa (Guerranti et al., 2002; Scirè et al., 2011; Hope-Onyekwere et al., 2012). On the other hand, the direct inhibitory action of MPE is probably caused by L-dopa, the main bioactive component, which acts in synergy with other compounds.
Anti-microbial properties of Mucuna pruriens leaves
Various parts of certain plants are known to contain substances that can be used for therapeutic purposes or as precursors for the production of useful drugs (Sofowora, 1982). Plant-based anti-microbials represent a vast untapped source of medicines and further investigation of plant anti-microbials is needed. Anti-microbials of plant origin have enormous therapeutic potential. Phytochemical compounds are reportedly responsible for the anti-microbial properties of certain plants (Mandal et al., 2005). While bioactive compounds are often extracted from whole plants, the concentration of such compounds within the different parts of the plant varies. Parts known to contain the highest concentration of the compounds are preferred for therapeutic purposes. Some of these active components operate individually, others in combination, to inhibit the life processes of microbes, particularly pathogens. Crude methanolic extracts of M. pruriens leaves have been shown to have mild activity against some bacteria in experimental settings ( Table 1 ), probably due to the presence of phenols and tannins (Ogundare and Olorunfemi, 2007). Further studies are required in order to isolate the bioactive components responsible for the observed anti-microbial activity.
Neuroprotective effect of Mucuna pruriens seeds
In India, the seeds of M. pruriens have traditionally been used as a nervine tonic, and as an aphrodisiac for male virility. The pods are anthelmintic, and the seeds are anti-inflammatory. Powdered seeds possess anti-parkinsonism properties, possibly due to the presence of L-dopa (a precursor of neurotransmitter dopamine). It is well known that dopamine is a neurotransmitter. The dopamine content in brain tissue is reduced when the conversion of tyrosine to L-dopa is blocked. L-Dopa, the precursor of dopamine, can cross the blood-brain barrier and undergo conversion to dopamine, restoring neurotransmission (Kulhalli, 1999). Good yields of L-dopa can be extracted from M. pruriens seeds ( Table 1 ) with EtOH-H2O (1:1), using ascorbic acid as a protector (Misra and Wagner, 2007). An n-propanol extract of M. pruriens seeds yields the highest response in neuroprotective testing involving the growth and survival of DA neurons in culture. Interestingly, n-propanol extracts, which contain a negligible amount of L-dopa, have shown significant neuroprotective activity, suggesting that a whole extract of M. pruriens seeds could be superior to pure L-dopa with regard to the treatment of parkinsonism.
Anti-diabetic effect of Mucuna pruriens seeds
Using a combination of chromatographic and NMR techniques, the presence of d-chiro-inositol and its two galacto-derivatives, O-α-d-galactopyranosil-(1→2)-d-chiro-inositol (FP1) and O-α-d-galactopyranosil-(1→6)-O-α-d-galactopyranosil-(1→2)-D-chiro-inositol (FP2), was demonstrated in M. pruriens seeds (Donati et al., 2005). Galactopyranosyl d-chiro-inositols are relatively rare and have been isolated recently from the seeds of certain plants; they constitute a minor component of the sucrose fraction of Glycine max (Fabaceae) and lupins, and a major component of Fagopyrum esculentum (Polygonaceae) (Horbovitz et al., 1998). Although usually ignored in phytochemical analyses conducted for dietary purposes, the presence of these cyclitols is of interest due to the insulin-mimetic effect of d-chiro-inositol, which constitutes a novel signaling system for the control of glucose metabolism (Larner et al., 1998; Ortmeyer et al., 1995). According to Anktar et al., (1990), M. pruriens seeds used at a dose of 500 mg/kg reduced plasma glucose levels. These and other data demonstrated that the amount of seeds necessary to obtain a significant anti-diabetic effect contain a total of approximately 7 mg of d-chiro-inositol (including both free, and that derived from the hydrolysis of FP1 and FP2). The anti-diabetic properties of M. pruriens seed EtOH/H2O 1:1 extract are most likely due to d-chiro-inositol and its galacto-derivatives ( Table 1 ).
Anti-oxidant activity of Mucuna pruriens
Free radicals that have one or more unpaired electrons are produced during normal and pathological cell metabolism. Reactive oxygen species (ROS) react readily with free radicals to become radicals themselves. Anti-oxidants provide protection to living organisms from damage caused by uncontrolled production of ROS and concomitant lipid peroxidation, protein damage and DNA strand breakage. Several substances from natural sources have been shown to contain anti-oxidants and are under study. Anti-oxidant compounds such as phenolic acids, polyphenols, and flavonoids, scavenge free radicals such as peroxide, hydroperoxide or lipid peroxyl, and thus inhibit oxidative mechanisms. Polyphenols are important phytochemicals due to their free radical scavenging and in vivo biological activities (Bravo, 1998); the total polyphenolic content has been tested using Folin-Ciocalteau reagent. Flavonoids are simple phenolic compounds that have been reported to possess a wide spectrum of biochemical properties, including anti-oxidant, anti-mutagenic and anti-carcinogenic activity (Beta et al., 2005). The hydrogen donating ability of the methanol extract of M. pruriens was measured in the presence of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical. In a recent study, Kottai Muthu et al. (2010) found that ethylacetate and methanolic extract of whole M. pruriens plant (MEMP), which contains large amounts of phenolic compounds, exhibits high anti-oxidant and free radical scavenging activities. These in vitro assays indicate that this plant extract is a significant source of natural anti-oxidant, which may be useful in preventing various oxidative stresses. It has been reported (Ujowundu et al., 2010) that methanolic extracts of M. pruriens leaves have numerous biochemical and physiological activities, and contain pharmaceutically valuable compounds ( Table 1 ).
Possible usage of Mucuna pruriens for skin treatments
The skin is one of the main targets of several exogenous insults such as UV radiation, O3, and cigarette smoke, and all of these exert toxicity via the induction of oxidative stress (Valacchi et al., 2000). Several skin pathologies, such as psoriasis, dermatitis, and eczema, are related to increased oxidative stress and ROS production (Briganti and Picardo, 2003), and research investigating novel natural compounds with anti-oxidant proprieties is an expanding field. As mentioned above, certain plant-derived compounds have been an important source of traditional treatments for various diseases, and have received considerable attention in more recent years due to their numerous pharmacological proprieties.
Recent preliminary studies from our group have shown that human keratinocytes treated with a methanolic extract from MP leaves exhibit downregulation of total protein expression. In addition, treatment with MP significantly decreased the baseline levels of 4HNE present in human keratinocytes (Lampariello et al., 2011). This preliminary study suggests that evaluating the effect that topical MP methanolic extract treatment may have on skin diseases would be worthwhile, as would further work aimed at clarifying the mechanisms involved in such effects.
Mucuna pruriens is an exceptional plant. On the one hand it is a good source of food, as it is rich in crude protein, essential fatty acids, starch content, and certain essential amino acids. On the other hand, it also contains various anti-nutritional factors, such as protease inhibitors, total phenolics, oligosaccharides (raffinose, stachyose, verbascose), and some cyclitols with anti-diabetic effects. In fact, all parts of the Mucuna plant possess medicinal properties. The main phenolic compound is L-dopa (5%), and M. pruriens seeds contain some components that are able to inhibit snake venom. In addition, methanolic extracts of M. pruriens leaves have demonstrated anti-microbial and anti-oxidant activities in the presence of bioactive compounds such as phenols, polyphenols and tannins, and preliminary studies on keratinocytes support its possible topical usage to treat redox-driven skin diseases. Collectively, the studies cited in this review suggest that this plant and its extracts may be of therapeutic value with regard to several pathologies, although further work is needed to investigate in more detail the mechanisms underlying the pharmacological activities of MP.
The Magic Velvet Bean of Mucuna pruriens Lucia Raffaella Lampariello 1 Department of Chemistry, University of Siena, Italy. Alessio Cortelazzo 2 Department of Internal Medicine,